Proteolipid Protein 2 (PLP2) ELISA Kit

Proteolipid Protein 2 (PLP2) ELISA Kit: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm

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SPECIFICATIONS

Catalog Number

ABIN422085

Size

96 tests

Antigen

Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2)

Gene Id

5355

Accession

Q04941

Assay Range

0.156-10 ng/mL

Sample Type

Tissue Homogenate,Biological Fluids

Sensitivity

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero standard added by their three standard deviations.

Entrez Gene Id

5355