Prouroguanylin, Recombinant, Human

Prouroguanylin is a 112-amino-acid prohormone precursor of uroguanylin- mature biologically active 16-amino-acid peptide cleaved from its C-terminus. Guanylin binds to receptor-guanylate cyclases (CG-C) resulting in increased intracellular cGMP levels leading to CFTCR (Cystic Fibrosis Transmembrane Conductance Regulator) activation and subsequent rise in fluid and electrolyte secretion into the lumen. Prouroguanyline knock-out mice showed impaired renal excretion of external NaCl leasing to elevated blood pressure independent of the level of diatary salt intake. Prouroguanylin is the predominant circulating molecular form found in circulation and in biological fluids.\n\nDescription: \nThe Recombinant Human Prouroguanylin is 10kD protein containing 88 amino acid residues of the human prouroguanylin and 2 additional amino acid residues.\n\nAmino Acid Sequence: \nGPVYIQYQGF RVQLESMKKL SDLEAQWAPS PRLQAQSLLP AVCHHPALPQ DLQPVCASQE ASSIFKTLRT IANDDCELCV NVACTGCL\n\nSpecificity: \nThe recombinant sequnce is 100% homologous to the native human sequence.\n\nApplications: \nWestern blotting, ELISA\n\nReconstitution: \nAdd 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.\n\nStability/Shelf Life: \nStore lyophilized protein at -20 degrees C. Aliquot the product after reconstitution to avoid repeated thaw-freeze cycles and store at -80 degrees C. Reconstituted protein can be stored at 4 degrees C for a limited period of time. The lyophilized protein remains stable until the expiry date when stored at -20 degrees C.

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SPECIFICATIONS

Catalog Number

P9119-90

Size

2ug

Form

Supplied as a lyophilized powder in 50mM phosphate buffer, pH 7.4.

Purity

(same/more than) 95% (SDS-PAGE and HPLC analyzed); Three-step procedure using IMAC and size exclusion chromatography before and after refolding.

References

Cui L, Blanchard RK, Coy LM, Cousins RJ. Prouroguanylin overproduction and localization in the intestine of zinc-deficient rats. J Nutr. 2000 Nov;130(11):2726-32.