PSG-1, Human BioAssay(TM) ELISA Kit (B1G1, CD66f, DHFRP2, PBG1, PSbG1, PSG95, PSGGA)

The PSG-1, Human BioAssay(TM) ELISA Kit Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PSG-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human PSG-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PSG-1 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PSG-1.\n\nIntended Use:\nThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal\nantibody specific for PSG-1 has been pre-coated onto a microplate. Standards and samples are\npipetted into the wells and any PSG-1 present is bound by the immobilized antibody. After\nwashing away any unbound substances, an enzyme-linked monoclonal antibody specific for\nPSG-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme\nreagent, a substrate solution is added to the wells and color develops in proportion to the\namount of PSG-1 bound in the initial step. The color development is stopped and the intensity\nof the color is measured.\n\nSpecificity:\nRecognizes natural and recombinant human PSG-1. \n\nSensitivity:\n~0.48ng/ml\n\nRange: \n~3.13-200ng/ml (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)\n\nKit Components:\nPSG-1 Microplate, 1x96wells \nPSG-1 Conjugate, 1x21ml\nPSG-1 Standard, 1x1000ng\nAssay Diluent, 1x11ml\nCalibrator Diluent, 1x21ml\nWash Buffer Concentrate, 1x21ml\nColor Reagent A, 1x12ml\nColor Reagent B, 1x12ml\nStop Solution, 1x6ml\nPlate Sealers, 1x4 adhesive strips\n\nStorage and Stability:\nStore other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145764

Size

1Kit

Applications

ELISA

References

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