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Psophocarpus tetragonolobus Lectin II (PTL II, WBA II)

Cat no: P9176-75C

Psophocarpus tetragonolobus Lectin II (PTL II, WBA II)

PTL II is an acidic dimeric glycoprotein with a molecular weight of about 54,000. Subunits can be dissociated in the absence of reducing agents and migrate in SDS-PAGE as a single band. The isoelectric point of this lectin is about pH 5.5. Like PTL I, this lectin binds preferentially to galactosides, with N-acetylgalactosamine being the most inhibitory monosaccharide. However, in contrast to PTL I, this lectin prefers the beta anomeric configuration. PTL II shows a high affinity toward blood group H structures and the T-antigen. In general, though, PTL II has lower affinities for inhibiting sugars than does PTL I. PTL I and PTL II also differ in their erythrocyte agglutinating properties. Tissue staining results indicate preference for some vascular elements with overall lighter staining than obtained with PTL I.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nInhibiting Sugar: \n100mM N-acetylgalactosamine\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P9176-75C

Size

5mg

Applications

ELISA

Form

Supplied as a powder in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide, 20mM lactose. Reconstitute with 1ml dH2O.

Alternative Names

Winged Bean, Goa Bean, Asparagus Pea, Winged Pea

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