PTF1A (Pancreas Transcription Factor 1 Subunit alpha, Class A Basic Helix-loop-helix protein 29, bHLHa29, Pancreas-specific Transcription Factor 1a, bHLH Transcription Factor p48, p48 DNA-binding Subunit of Transcription Factor PTF1, PTF1-p48, BHLHA29, PT

PTF1A (Pancreas transcription factor 1a; also p48 and bHLHA29) is a cell specific member of the bHLH family of proteins. Although its predicted MW is 41kD, a high ratio of Pro, Leu and Ala increases its MW to 48kD in SDS-PAGE. PTF1A forms a trimeric transcription factor complex with a p75 subunit (HEB, E2 or E47) and a p64 subunit (usually RBP-L). Within this complex, unfortunately termed PTF1, p48/PTF1A binds to a DNA E-box while p64 binds to a TC-box. This regulates the gene transcription of about 20 enzymes synthesized by pancreatic acinar cells. Human PTF1A is 328aa in length. It contains one basic DNA binding HLH motif (aa150-175) and a dimerization interface (aa178-219). Overaa1-92, human PTF1A shares 88% aa identity with mouse PTF1A.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.5-1ug/ml\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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