PTGES2, Recombinant, Human, aa88-377, His-tag (C9orf15, GBF1, MPGES2, Membrane-associated Prostaglandin E Synthase)

PTGES2, also known as membrane-associated prostaglandin E synthase (mPGES) catalyzes the conversion of prostaglandin H2 (PGH2) to prostaglandin E2 (PGE2). Its N-terminal domain anchors it in the Golgi membrane, and its catalytic domain resides in the cytoplasm. Proteolysis within the cell generates a truncated soluble form beginning with amino acid 88 that resides in the cytosol (1). PTGES2 production of PGE2 is coupled to both COX-1 and COX-2 (2). PTGES2 is expressed in many tissues, with highest expression in brain, heart, skeletal muscle, kidney, and liver (1). In addition to its PGE2 production, PTGES2 activates transcription regulated by a gamma-interferon-activated transcription element (3). The protein may also play a role in colorectal cancer progression (4).\n\nSource:\nRecombinant protein corresponding to aa88-377 from human PTGES2, fused to 6-his tag at N-terminal, expressed in E. coli.\n\nMolecular Weight: \n~28-32kD\n\nEndotoxin:\n<1.0EU/1ug (LAL method).\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145829

Size

20ug

Form

Supplied as a liquid in Tris, NaCl, DTT, Glycerol.

Purity

~85% (SDS-PAGE)

References

1. Tanikawa, N. et al. (2002) Biochem. Biophys. Res. Commun. 291:884. 2. Murakami, M. et al. (2003) J. Biol. Chem. 278:37937. 3. Hu, J. et al. (2002) J. Biol. Chem. 277:30253. 4. Seo, T. et al. (2009) Virchows Arch. 454:667.