PTP1B (Tyrosine-protein Phosphatase Non-receptor Type 1, Protein-tyrosine Phosphatase 1B, PTP-1B, PTPN1, PTP1B)

PTP1B is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000 \nWestern Blot: 1:100-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9109-03C

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Ragab, A., et al., J. Biol. Chem. 278(42):40923-40932 (2003). 2. Sun, J.P., et al., J. Biol. Chem. 278(14):12406-12414 (2003). 3. Boute, N., et al., EMBO Rep. 4(3):313-319 (2003). 4. Li, S., et al., Arch. Biochem.Biophys. 410(2):269-279 (2003). 5. Yigzaw, Y., et al., J. Biol. Chem. 278(1):284-288 (2003).

Additional Info

Recognizes human PTP1b. Species Crossreactivity: mouse.