(Biotin-Capture) Avidin beads are useful carriers for biotinylated molecules, in various applications such as the construction of affinity columns or for conducting immuno-precipitations (IP). Biotin-Capture Beads contain modified Avidin that is immobilized on rigid, highly cross-linked beaded agarose possessing high chemical Stability. Modified Avidin provides a substantial improvement over native Avidin. The high affinity to biotin is maintained, while background problems are minimized due to chemical modification on the protein. The Biotin-Capture Beads has proven to be particularly useful in the isolation of antigens and nucleic acids by employing biotinylated antibodies, or nucleic acid probes respectively. Biotin-Capture Beads can be used in numerous additional affinity chromatography applications.
Biotin-Capture Beads Specifications:
Matrix: Sepharose CL-4B
Coupling method: TargetLock
Type of Avidin bound to beads: Modified Avidin
Binding capacity: 2-4mg biotinilated BSA per ml of beads
Bead size: 40-165um
Bead structure: Highly cross-linked spherical agarose, 4%
Max back pressure: 0.3MPa, 3 bar
Max. flow rates: 4ml/min/cm2
Recommended flow rate: 1-2ml/min/cm2
Stability of the matrix: pH 2-11.
Storage: 4 degrees C in PBS pH 7.4 added with sodium azide 0.05% (w/v) as a preservative.
Storage:
Store gel refrigerated in phosphate buffered saline (PBS) or other ligand-compatible
buffer added with 0.1% azide (w/v) as a preservative until use.
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