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Purification Media, Avidin Beads, BioSeparation(TM)

Cat no: P9100-05

Purification Media, Avidin Beads, BioSeparation(TM)

(Biotin-Capture) Avidin beads are useful carriers for biotinylated molecules, in various applications such as the construction of affinity columns or for conducting immuno-precipitations (IP). Biotin-Capture Beads contain modified Avidin that is immobilized on rigid, highly cross-linked beaded agarose possessing high chemical Stability. Modified Avidin provides a substantial improvement over native Avidin. The high affinity to biotin is maintained, while background problems are minimized due to chemical modification on the protein. The Biotin-Capture Beads has proven to be particularly useful in the isolation of antigens and nucleic acids by employing biotinylated antibodies, or nucleic acid probes respectively. Biotin-Capture Beads can be used in numerous additional affinity chromatography applications.\n\nBiotin-Capture Beads Specifications:\nMatrix: Sepharose CL-4B\nCoupling method: TargetLock\nType of Avidin bound to beads: Modified Avidin\nBinding capacity: 2-4mg biotinilated BSA per ml of beads\nBead size: 40-165um\nBead structure: Highly cross-linked spherical agarose, 4%\nMax back pressure: 0.3MPa, 3 bar\nMax. flow rates: 4ml/min/cm2\nRecommended flow rate: 1-2ml/min/cm2\nStability of the matrix: pH 2-11.\nStorage: 4 degrees C in PBS pH 7.4 added with sodium azide 0.05% (w/v) as a preservative.\n\nStorage:\nStore gel refrigerated in phosphate buffered saline (PBS) or other ligand-compatible\nbuffer added with 0.1% azide (w/v) as a preservative until use.

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SPECIFICATIONS

Catalog Number

P9100-05

Size

1ml

Read more on Supplier website

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Ch/Bird

More info

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, NHP

More info
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