Protein G originated from Streptococci is a monomeric protein with a molecular weight of about 50,000kD. Protein G binds with high affinity to the Fc region of most classes and subclasses of immunoglobulins from mouse, rabbit, goat and human. Due to this binding ability, Protein G is commonly used in the purification and recovery of either polyclonal or monoclonal immunoglobulins. Recombinant Protein G used in Protein G beads is a genetically engineered form of Protein G. Non-essential regions have been removed while leaving the IgG binding sites intact. The recombinant Protein G has been immobilized on Sepharose beads in order to create a versatile product for both the purification of various mammalian immunoglobulins and for isolating immune complexes by immunoprecipitation (IP).
Matrix:
SepharoseTM CL-4B
Coupling Method: TargetLock
Protein G Density: 3-4mg/ml of gel
Binding Capacity:
~4-8mg rabbit IgG per ml of beads
Mean Bead Size:
40-165um
Bead Structure:
Highly cross-linked spherical agarose, 4%
Max Back Pressure:
0.3MPa, 3 bar
Max. Flow Rates: 4ml/min/cm2
Recommended Flow Rate: 1-3 ml/min/cm2
Stability of the Matrix:
pH 2-11.
Storage:
Store gel refrigerated at 4 degrees C in PBS pH 7.4 containing 0.1% sodium azide as a preservative, or PBS pH 7.4 containing 20% ethanol.
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