Gelatin affinity media is commonly used for the purification of various proteins, mainly serum proteins, by affinity chromatography. Gelatin Beads are made with highly purified Bovine Gelatin and capable of purifying human fibronectin.
Gelatin Beads Specifications
Matrix: Sepharose CL-4B
Coupling method: TargetLock.
Gelatin density: 2-3mg/ml of gel
Binding capacity: ~1mg of Fibronectin
Mean bead size: 40 -165um
Bead structure: Highly cross-linked spherical agarose, 4%
Max back pressure: 0.3MPa, 3 bar
Max. flow rates: 4ml/min/cm2
Recommended flow rate: 1-3ml/min/cm2
Storage:
Store gel refrigerated in phosphate buffered saline (PBS) or other ligand-compatible
buffer added with 0.1% azide as a preservative until use.
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