Glutathione Beads are used for the purification of glutathione S- transferase (GST) tagged proteins, produced using various commercial expression vectors. GST-fused proteins can be purified from bacterial lysates by one-step affinity purification. Glutathione Beads is state of the art affinity-resin product that consists of glutathione attached through sulfur to an epoxy-activated 4% cross-linked beaded agarose. The use of the Glutathione Beads enables the purification of various GST-fused proteins, while maintaining mild, non-denaturing conditions throughout the purification process.
Glutathione Beads characteristics:
Matrix: Sepharose CL-4B
Activation method: Oxiran
Binding capacity: 4-8mg/ml recombinant GST
Bead size: 40-165um
Bead structure: Highly cross-linked spherical agarose, 4%
Max back pressure: 0.3MPa, 3bar
Max. flow rate: 4ml/min/cm2
Recommended flow rate: 1-2ml/min/cm2
Stability of the matrix: pH 4-10
Storage and Stability:
Store at 4 degrees C in PBS pH 7.4 with with sodium azide 0.1% (w/v) added as preservative.
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