Heparin Beads are made by coupling 20-35kD size Heparin extracted from Porcine intestinal mucosa (Type I) to Sepharose beads. These beads are useful for isolating heparin-binding protein such as Antithrombin III, lipoproteins, various enzymes and DNA binding proteins.
Heparin Beads Specifications:
Matrix: Sepharose CL-4B
Coupling method: TargetLock.
Heparin density: 0.7-1.2mg/ml of 20-35kD Heparin. Thrombin binding capacity minimum 0.7mg/ml of beads
Mean bead size: 40 -165um
Range of pH: 3.0-11.0
Bead structure: Highly cross-linked spherical agarose, 4%
Max back pressure: 0.3MPa, 3 bar
Max. flow rates: 4ml/min/cm2
Recommended flow rate: 1-3ml/min/cm2
Storage and Stability:
May be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
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