Ig-Thio-Capture Beads absorbent is a chemical based immunoadsorbent aimed for the purification of immunoglobulins. Ig-Thio-Capture Beads support is based on the ability of some proteins, especially immunoglobulins, to bind to an immobilized chemical ligand that contains a sulfone group in close proximity to a thioether. The Ig-Thio-Capture Beads also termed thiophilic adsorption chromatography provides a low cost, efficient alternative to other antibody purification methods. The purification with thiophilic gel is a simple, rapid, one step purification method for antibody purification from serum, ascites or tissue culture supernatant. In addition, the method exhibits good protein recovery with excellent preservation of antibody activity due to gentle elution conditions, which usually yield concentrated, essentially salt-free, highly purified immunoglobulins at near neutral pH. The gel has a high binding capacity and it has a broad specificity toward immunoglobulins derived from various animal species irrespective of the type of immunoglobulin or the immunoglobulin subclass.
Ig-Thio-Capture Beads characteristics:
Matrix: Sepharose CL-4B
Activation method: divinyl sulfone.
Chelating group: sulfone group in proximity to a thioether group
Binding capacity: ~8-12mg IgG per ml of beads
Mean bead size: 40-165um
Bead structure: Highly cross-linked spherical agarose, 6%
Max back pressure: 0.3MPa, 3 bar
Max. flow rates: 4ml/min/cm2
Recommended flow rate: 1-3ml/min/cm2
Stability of the matrix: pH 3-13.
Storage: 4 degrees C in PBS pH 7.4 added with sodium azide 0.1% (w/v) as a preservative.
Storage:
Store gel refrigerated in phosphate buffered saline (PBS) or other ligand-compatible
buffer added with 0.1% azide (w/v) as a preservative until use.
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