Activated KLH and BSA are commonly used as protein carriers for haptens such as peptides in order to enable the immune response to small molecules. In many cases KLH conjugate is used for the immunization, while BSA conjugate is used in immunoassys of the resulting antibodies. BSA conjugates are used in order to filter out the antibodies directed to the KLH. However in some cases BSA or other proteins are preffered as carrier proteins because of a variety of other reasons. The Maleimide activated KLH and BSA are preactivated with a heterobifunctional cross-linker (GMBS). These activated proteins may be reacted with biomolecules that contain a free sulfhydryl groups, to form a stable thioether bond.
Activation Method: GMBS
Binding Capacity: ~2.5-4mg of peptide (average MW of 1000-2500) per 2mg BSA
Protein Concentration: 4mg/ml (0.5ml)
Storage Buffer: PBS pH 7.5
Storage and Stability:
Stable for 3 days refrigerated
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