Activated KLH and BSA are commonly used as protein carriers for haptens such as peptides, in order to enable the immune response to these small molecules. KLH is most popular for immunization while BSA conjugate is used in immunoassays of the resulting antibodies in order to filter out the anti KLH response. The Maleimide activated KLH and BSA are preactivated with a heterobifunctional cross-linker (GMBS). These activated proteins can be reacted with compounds that contain a free sulfhydryl group to form a stable thioether bond.
Activation method: GMBS
Binding Capacity: ~2-4mg of peptide (average MW of 1000-2500) per 2mg KLH
Protein Concentration: 4mg/ml (0.5ml)
Storage Buffer: PBS pH 7.5
Storage and Stability:
Stable for 3 days refrigerated
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