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Purified anti-Akt Phospho (Ser473)

Purified anti-Akt Phospho (Ser473)

Cat no: 649002

Supplier: BioLegend
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Akt (also known as protein kinase B alpha) is a 60 kD serine/threonine specific kinase containing a pleckstrin domain. Akt plays a critical role in controlling survival and apoptosis. This kinase is ubiquitously expressed and translocates to the membrane upon activation. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI 3-kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2, responsible for phosphorylation of Akt at Ser473, has been identified as a mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1. Activated Akt can then phosphorylate a wide range of substrates including transcription factors (e.g. FOXO1), kinases (GSK-3,Raf-1, ASK, Chk1) and other proteins with important signaling roles (e.g. Bad, MDM2). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and by inactivation of several targets, including Bad, forkhead transcription factor, c-Raf and caspase-9. Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex. The phosphor-Akt (S473) antibody specifically detects phosphorlated Akt at the Ser473 site.
Catalogue number: 649002
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunofluorescence, Western Blot
Size: 200 microl
Clone: Poly6490
Gene: 207
Dilutions: Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 10 microg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store frozen at -20C.
Antigen: Modified Synthetic peptide
Isotype: Rabbit Polyclonal
Clonality: Polyclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: -20 C
Alternative names: Protein kinase B alpha (PRK-BA), Serine/Threonine specific kinase RAC alpha, Protein kinase Akt
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