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Purified anti-AMPKalpha

Purified anti-AMPKalpha

Cat no: 649801

Supplier: BioLegend
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AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. AMPK is a heterodimeric protein serine/threonine kinase that is composed of alpha- (catalytic) and beta/gamma- (regulatory) subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. This catalytic subunit contains a classical serine/threonine protein kinase domain close to the N-terminal. Free catalytic alpha subunits are usually inactive due to the presence of an autoinhibitory domain that is located in the center of this subunit. When the alpha subunit forms a functional complex with the beta and gamma subunits, this autoinhibitory function no longer blocks the catalytic function. In response to cellular metabolic stresses, AMPK is activated in an AMP-dependent manner by targeted phosphorylation of Thr172 in the alpha subunit by the upstream kinase like LKB1, calmodulin-dependent protein kinase kinases and transforming growth factor-beta-activated kinase-1. Activated AMPK phosphorylates many metabolic enzymes to stimulate catabolic pathways, such as ketogenesis, and inhibit anabolic pathways, such as protein synthesis. Recent studies also suggest the important role of AMPK in regulating chemotaxis and cytokine secretion in immune cells.
Catalogue number: 649801
Reactivities: Human
Hosts: Rabbit
Applications: Western Blot
Size: 50 microl
Clone: Poly6498
Gene: 5564
Dilutions: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store undiluted at -20C.
Antigen: AMPK catalytic subunit (alpha subunit)
Isotype: Rabbit IgG
Clonality: Polyclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: -20 C
Alternative names: AMPK, AMP-activated protein kinase, 5' adenosine monophosphate-activated protein kinase, 5
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