Home  >  Products  >  Purified anti-APC3
Purified anti-APC3

Purified anti-APC3

Cat no: 610901

Supplier: BioLegend
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
APC3 (anaphase-promoting complex subunit 3) is a member of the E3 enzyme family. This protein contains TPR repeats and has a molecular weight of approximately 95 kD. The APC3 protein is located at the spindle poles, the spindle microtubules, kinetochores and chromosome arms. This protein is required for core stability and functions as a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC3 protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1. The APC3 protein interacts with Rb, Mad2, p55CDC, BUBR1, as well as the APC complex proteins noted above. The APC3 protein can be modified by phosphorylation by cdk1-cyclin B. The Poly6109 antibody has been shown to be useful for Western blotting of the human APC3 protein.
Catalogue number: 610901
Reactivities: Human
Hosts: Rabbit
Applications: Immunofluorescence, Western Blot
Size: 50 microl
Clone: Poly6109
Gene: 324
Dilutions: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store frozen at -20 C.
Antigen: Recombinant (partial), N-terminal
Isotype: Rabbit Polyclonal
Clonality: Polyclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: -20 C
Alternative names: Anaphase-promoting complex subunit 3, Cell division cycle protein 27 (Cdc27), Nuc2
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave