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Purified anti-Aurora A (Aurora 2)

Purified anti-Aurora A (Aurora 2)

Cat no: 603301

Supplier: BioLegend
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Aurora A (also known as Aurora 2) is a serine/threonine kinase with a molecular weight of approximately 46 kD. This kinase is highly expressed in the thymus and some tumors and is also expressed in other tissues including the lung, testis, colon, placenta, and fetal liver. Aurora A localizes in the midzone or central spindle in late anaphase and is concentrated in the midbody in telophase and during cytokinesis. This kinase is believed to act in cell cycle regulation during anaphase and/or telophase at centrosome/spindle pole during chromosome segregation. Aurora A has been shown to regulate cleavage of polar spindle microtubules at the onset of cytokinesis during mitosis. Defects in Aurora A cause numerous centrosome aberrations including aneuploidy (genetic variant with amino acid substitution F31I). Aurora A expression is cell cycle regulated, low in G1/S, and accumulating in G2/M. Expression is upregulated in cancer cells during M phase. Phosphorylation by PKA has been shown to regulate function. Aurora A phosphorylation has been reported on Thr 288. This kinase associates with the centrosome and mitotic spindles, NM23-H1, protein phosphatase type I, and co-localizes with gamma-tubulin. The Phe 31 variant has been shown to interact with the E2 ubiquitin-conjugating enzyme, UBE2N. The Poly6033 antibody recognizes the N-terminal region of Aurora A. The Poly6033 antibody has been shown to be useful for Western blotting of human and mouse Aurora A protein and for immunoprecipitation of human Aurora A (mouse not tested).
Catalogue number: 603301
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunofluorescence, Western Blot
Size: 50 microl
Clone: Poly6033
Gene: 6790
Dilutions: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store frozen at -20 C.
Antigen: Recombinant (partial), N-terminal
Isotype: Rabbit Polyclonal
Clonality: Polyclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: -20 C
Alternative names: Aurora 2, Aurora- and IPll-like kinase (AIK), Serine/threonine kinase 15, serine/threonine kinase 6
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