Purified anti-Bax

Bax is a 21 kD pro-apoptotic protein known to regulate apoptosis. Bax is found in the cytoplasm, mitochondria, and nucleus and is highly expressed in hematopoietic stem cells, the ovary, and in the lymph node. Bax binds the anti-apoptotic protein Bcl-2 as a heterodimer or forms homodimers. The relative levels of pro-apoptotic proteins such as Bax and anti-apoptotic proteins such as Bcl-2 determines whether cell death will occur following an apoptotic stimulus. Bax accelerates the opening of mitochondrial VDAC altering membrane potential and allowing cytochrome c to pass out of the mitochondria into the cytosol to initiate downstream caspase activation. p53 can transcriptionally activate the Bax gene to induce apoptosis. Bax has been shown to be mutated in some human cancers. Clone 5B7 has been shown to be useful for western blotting and immunoprecipitation of the mouse Bax protein.This antibody does not cross-react with Bcl-2 or Bcl-XL proteins.

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SPECIFICATIONS

Catalog Number

633701

Size

25 microg

Applications

IP, WB

Hosts

Mouse

Reactivities

Mouse

Clone

5B7

Antigen

Amino acids: 8-16 of murine Bax protein

Gene Id

581

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 microg per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 microg/ml. It is recommended that the reagent be titrated for optimal performance for each application.>

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store undiluted at at 4C.

Alternative Names

BCL2 associated X protein, apoptosis regulator Bax

Regulatory Status

RUO