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Purified anti-Bcl-2

Purified anti-Bcl-2

Cat no: 633502

Supplier: BioLegend
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Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist alpha=25 kD; beta=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, intracellular membrane nuclear envelope, and endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Bcl-2 is modified by phosphorylation and ubiquitination. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.
Catalogue number: 633502
Reactivities: Mouse, Rat
Hosts: Mouse
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 100 microg
Clone: BCL/10C4
Gene: 596
Dilutions: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 microg per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 microg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store undiluted at at 4C.
Concentration: 0.5 mg/ml
Antigen: N-terminal, Amino acid residues 61-76 of mouse Bcl-2
Isotype: Mouse IgG1, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: B-cell leukemia 2
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