Purified anti-beta-Actin

Cat no: 622101

Supplier: BioLegend

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Description
Additional Information

beta-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting). The Poly6221 antibody has been shown to be useful for Western blotting of mouse, rat, and human beta-actin.

Catalogue number:	622101
Reactivities:	Human, Mouse, Rat
Hosts:	Rabbit
Applications:	Immunofluorescence, Immunohistochemistry, Western Blot
Size:	50 microl
Clone:	Poly6221
Gene:	11461
Dilutions:	Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. For IHC, use a 1:100 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer:	Upon receipt, store frozen at -20 C.
Antigen:	Peptide-KLH, NH2 terminus
Isotype:	Rabbit Polyclonal
Clonality:	Polyclonal
Regulatory status:	RUO
Shipping temp:	Ambient RT
Storage temp:	-20 C
Alternative names:	Actin, cytoplasmic 1