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Purified anti-Caspase-3

Purified anti-Caspase-3

Cat no: 622701

Supplier: BioLegend
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Caspase 3 (also known as cpp32, Apopain, Yama, and PARP cleavage protease) is a member of the peptidase family C14. This cytoplasmic caspase functions as a heterotetramer and has two isoforms (one acts as a dominant negative inhibitor). The pro-form of this caspase is approximately 32 kD. Caspase 3 is involved in the caspase activation cascade responsible for apoptosis execution. This caspase cleaves/activates SREBPs, Caspase 6, Caspase 7, and Caspase 9. Caspase 3 is inhibited by c-IAP1, c-IAP2, XIAP, survivin, and Livin and activated by Caspase 6, -8 , and -10, granzyme B, Apaf-1 by cleavage into large p17 and small p12 subunits. Active heterodimers form between the large p17 subunit and small p11 subunit of Caspase 7 and vice versa. Caspase 3 has been shown to cleave PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topoisomerase I, vimentin, Rb, lamin B, mdm2, and beta-catenin. The 4-1-18 monoclonal antibody has been shown to be useful for Western blotting of human caspase 3 (32 kD pro-caspase and 17 kD cleaved caspase).
Catalogue number: 622701
Reactivities: Human
Hosts: Mouse
Applications: Western Blot
Size: 25 microg
Clone: 4-1-18
Gene: 12367
Dilutions: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store at 4C.
Concentration: 0.5 mg/ml
Antigen: Recombinant full length human protein
Isotype: Mouse IgG2a
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Caspase 3 preproprotein, Casp3, cpp32, Apopain, Yama, PARP cleavage protease, SREBP cleavage activity 1
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