Purified anti-Caspase 3

Caspase 3 is a member of the peptidase family C14. This cytoplasmic caspase functions as a heterotetramer and has two isoforms (one acts as a dominant negative inhibitor). The pro-form of this caspase is approximately 32 kD. Caspase 3 is involved in the caspase activation cascade responsible for apoptosis execution. Caspase 3 is inhibited by c-IAP1, c-IAP2, XIAP, survivin, and Livin and activated by Caspase 6, -8 , and -10, granzyme B, Apaf-1 by cleavage into large p17 and small p12 subunits. Caspase 3 has been shown to cleave PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topoisomerase I, vimentin, Rb, lamin B, mdm2, and beta-catenin. The poly6341 antibody has been shown to be useful for western blotting, immunoprecipitation and immunohistochemistry of human Human caspase-3 precursor.

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SPECIFICATIONS

Catalog Number

634101

Size

125 microl

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Clone

Poly6341

Antigen

Peptide mapping to the amino terminal domain of human caspase-3 prescursor

Gene Id

12367

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 25microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C. DO NOT FREEZE.

Alternative Names

Caspase 3 preproprotein, Casp3, cpp32, Apopain, Yama, PARP cleavage protease, SREBP cleavage activity 1

Regulatory Status

RUO