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Purified anti-Caspase-9

Purified anti-Caspase-9

Cat no: 621902

Supplier: BioLegend
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Caspase 9 (also known as ICE-like apoptotic protease 6 (ICE-LAP6), apoptotic protease Mch-6, and apoptotic protease activating factor 3 (Apaf-3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. Pro-Caspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, Bcl-XL, c-IAP1, c-IAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf-1/cytochrome c complex, and following cleavage by Apaf-1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 interacts with BIRC7 and has been shown to cleave PARP and vimentin. The 96-2-22 monoclonal antibody has been shown to be useful for Western blotting of human caspase 9 (46 kD pro-caspase 9 as well as the 34 kD cleaved caspase 9).
Catalogue number: 621902
Reactivities: Human
Hosts: Mouse
Applications: Immunoprecipitation, Western Blot
Size: 100 microg
Clone: 96-2-22
Gene: 842
Dilutions: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store at 4C.
Concentration: 0.5 mg/ml
Antigen: N-terminal fragment of human caspase-9
Isotype: Mouse IgG1, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Caspase-9 precursor, Casp9, ICE-like apoptotic protease 6 (ICE-LAP6), Apoptotic protease Mch-6, Apoptotic protease activating factor 3 (Apaf-3)
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