Purified anti-Cytochrome b-245 light chain

The integral membrane protein flavocytochrome b (Cyt b), a heterodimer formed by a 91-kD glycoprotein (heavy chain) and a 22-kD polypeptide (light chain), is the catalytic core of the phagocyte NADPH oxidase and generates superoxide which plays a critical role in host defense. The p22phox gene encodes the light alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. p22phox is a prerequisite for TNF-induced but not for Toll-like-receptor-induced ROS production. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD) that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells.

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SPECIFICATIONS

Catalog Number

650001

Size

25 microg

Applications

FC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Clone

44.1

Antigen

Partially purified human neutrophil flavocytochrome b

Gene Id

4519

Isotype

Mouse IgG2a, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody has been quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1 microg per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store undiluted at 4C.

Alternative Names

CYBA, cytochrome b-245 alpha polypeptide, p22phox, flavocytochrome b-558 alpha polypeptide

Regulatory Status

RUO