Purified anti-Cytochrome c

Cat no: 612301

Supplier: BioLegend

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Description
Additional Information

Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.

Catalogue number:	612301
Reactivities:	Human, Mouse, Rat
Hosts:	Mouse
Applications:	Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Size:	25 microg
Clone:	6H2.B4
Gene:	1355
Dilutions:	This antibody can be used at 2-4 microg /1 x107 cell equivalents for immunoprecipitation. For immunofluorescent staining, the suggested use of this reagent is ? 0.5 microg per 106 cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer:	Upon receipt, store at 4C.
Concentration:	0.5 mg/ml
Antigen:	Rat cyt c-OVA
Isotype:	Mouse IgG1, kappa
Clonality:	Monoclonal
Regulatory status:	RUO
Shipping temp:	Ambient RT
Storage temp:	4 C
Alternative names:	Cyt c