Purified anti-DNA-PKcs Phospho (Thr2609)

DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470 kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. Phosphorylated DNA-PKcs is upregulated after DNA damaage. The 10B1 monoclonal antibody recognizes phosphorylated human DNA-PKcs (Thr2609) and has been shown to be useful for immunofluorescence staining and Western blotting.

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SPECIFICATIONS

Catalog Number

612901

Size

25 microg

Applications

IF, WB

Hosts

Mouse

Reactivities

Hum

Clone

10B1

Antigen

Modified peptide

Gene Id

19090

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this DNA-PKcs antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1-4 microg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store undiluted at 4C.

Alternative Names

DNA-protein kinase catalytic subunit, DNA-activated protein kinase, DNA-dependent protein kinase

Regulatory Status

RUO