Purified anti-DNA-PKcs Phospho (Thr2609)

Cat no: 612902

Supplier: BioLegend

0 reviews | Write a Review Pencil

Description
Additional Information

DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470 kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. Phosphorylated DNA-PKcs is upregulated after DNA damaage. The 10B1 monoclonal antibody recognizes phosphorylated human DNA-PKcs (Thr2609) and has been shown to be useful for immunofluorescence staining and Western blotting.

Catalogue number:	612902
Reactivities:	Human
Hosts:	Mouse
Applications:	Immunofluorescence, Western Blot
Size:	100 microg
Clone:	10B1
Gene:	19090
Dilutions:	Each lot of this DNA-PKcs antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1-4 microg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer:	Upon receipt, store undiluted at 4C.
Concentration:	0.5 mg/ml
Antigen:	Modified peptide
Isotype:	Mouse IgG1, kappa
Clonality:	Monoclonal
Regulatory status:	RUO
Shipping temp:	Ambient RT
Storage temp:	4 C
Alternative names:	DNA-protein kinase catalytic subunit, DNA-activated protein kinase, DNA-dependent protein kinase