Purified anti-Eg5 Phospho (Thr927)

hEg5 (also known as kinesin-like protein KIF11, kinesin-related motor protein Eg5, kinesin-like spindle protein HKSP, and thyroid receptor interacting protein 5 (TRIP5)) is a 119 kD kinesin-like protein family, BimC subfamily. This protein is a catalytic kinesin motor with coiled-coil and Smc domains. hEg5 is localized at the centrosomes, spindle microtubules, and intracellular bridge. This motor protein is required for establishing the bipolar spindle. hEg5 is modified by phosphorylation on Thr927 by Cdc2 to allow association with the spindle apparatus. hEg5 has been shown to interact with the thyroid hormone receptor in presence of thyroid hormone and Cdc2. The Poly6205 antibody has been shown to react with phosphorylated human Eg5 (Thr927) by Western blot.

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SPECIFICATIONS

Catalog Number

620501

Size

50 microl

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum

Clone

Poly6205

Antigen

Modified peptide

Gene Id

3832

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store frozen at -20 C.

Alternative Names

Kinesin-like protein KIF11, kinesin-related motor protein Eg5, kinesin-like spindle protein HKSP, thyroid receptor interacting protein 5 (TRIP5)

Regulatory Status

RUO