Purified anti-eIF4E

Cat no: 606902

Supplier: BioLegend

0 reviews | Write a Review Pencil

Description
Additional Information

eIF4E (also known as eukaryotic translation initiation factor 4E) is a 25 kD member of the eukaryotic initiation factor 4E family. This ubiquitously expressed cytoplasmic protein recognizes and binds 7-methylguanosine containing mRNA "CAP" during initiation of protein synthesis. eIF4E facilitates ribosome binding by inducing unwinding of mRNA secondary structures. Activity of this protein is inhibited by binding of 4E-BP and PRH; expression is increased by H2O2 treatment. Activity can also be regulated by integrin alpha6beta4 ligation. Phosphorylation increases binding to mRNA CAPs and formation of eIF4F complex. eIF4E forms a trimeric complex with eIF4G, eIF4A (called eIF4F). Also associates with 4E-BP and PRH. The Poly6069 antibody has recognizes human eIF4E and has been shown to be useful for Western blotting.

Catalogue number:	606902
Reactivities:	Human
Hosts:	Rabbit
Applications:	Western Blot
Size:	200 microl
Clone:	Poly6069
Gene:	13684
Dilutions:	Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer:	Upon receipt, store frozen at -20 C.
Antigen:	Recombinant (partial), N-terminal
Isotype:	Rabbit Polyclonal
Clonality:	Polyclonal
Regulatory status:	RUO
Shipping temp:	Ambient RT
Storage temp:	-20 C
Alternative names:	Eukaryotic translation initiation factor 4E