Purified anti-FOXO3A (FKHRL1)

This polyclonal antibody recognizes human FOXO3A also known as forkhead box 03A, forkhead in rhabdomysacoma-like 1, and FKHRL1. FOXO3A is a member of the forkhead transcription factor family, containing a single forkhead domain, with a predicted molecular weight of approximately 71 kD. The FOXO3A protein is located in the cytoplasm when complexed to 14-3-3 protein; and in the nucleus when acting as a transcription factor for cell death genes. FOXO3A is widely expressed in the heart, brain, lung, liver, muscle, kidney, spleen, thymus, prostate, ovary, and small intestine. The FOXO3A protein triggers apoptosis by inducing cell death-specific genes. When survival factors are present, AKT1 phosphorylates FOXO3A leading to its association with 14-3-3 proteins and retention in the cytoplasm. Withdrawal of survival factors leads to dephosphorylation of FOXO3A and nuclear translocation which induces cell death. Overexpression of FOXO3A can cause growth suppression; while inhibition of FOXO3A has been reported to promote tumorigenesis under some conditions. FOXO3A acts at the G2/M checkpoint to activate DNA repair through the direct activation of proteins such as GADD45A. FOXO3A has also been shown to regulate erythroid differentiation. FOXO3A is modified at multiple phosphorylation sites (T32, S253, S315) and has been shown to interact with SMAD4, SMAD3, SMAD2, AKT1, 14-3-3 zeta, other proteins. This antibody has been shown to be useful for Western blotting.

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SPECIFICATIONS

Catalog Number

629801

Size

50 microl

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Clone

Poly6298

Antigen

Synthetic peptide

Gene Id

2309

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store frozen at -20 C.

Alternative Names

Forkhead box 03A, Forkhead in rhabdomysacoma-like 1, FKHRL1

Regulatory Status

RUO