Purified anti-GRP94

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. Glucose-regulated protein 94 (GRP94) is one of the most abundant endoplasmic reticulum (ER) resident proteins and is the ER counterpart of the cytoplasmic heat shock protein 90 (HSP90). GRP94 exists as homodimers containing an N-terminal ATP-binding domain, peptide-binding domain, and C-terminal dimerization domain. GRP94, a component of the GRP78 chaperone system in protein processing, has pro-survival properties with implicated function in cancer progression and autoimmune disease. In mouse tumor models, GRP94 derived from cancer cells also induces anti-tumor immune responses. One reason for this tumor immunogenicity is that GRP94 binds to the peptides from proteins in cancer cells and can therefore present these peptides as tumor antigens. Loss of function studies on GRP94 showed that it is required for embryonic development, regulation of toll-like receptors, and innate immunity of macrophages. Furthermore, GRP94 expression is strikingly high in dendritic cells, smooth muscle, and lung bronchial epithelium and has been shown to induce maturation of dendritic cells.

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SPECIFICATIONS

Catalog Number

649902

Size

100 microl

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Clone

Poly6499

Antigen

c-terminal recombinant protein

Gene Id

7184

Isotype

Rabbit IgG

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store undiluted at -20C.

Alternative Names

heat shock protein Hsp90 family protein, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, glucose regulated protein 94 kDa, tumor rejection antigen (gp96) 1

Regulatory Status

RUO