Purified anti-H2A.X Phospho (Ser139)

Cat no: 613402

Supplier: BioLegend

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Description
Additional Information

H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting and immunofluorescence.

Catalogue number:	613402
Reactivities:	Human
Hosts:	Mouse
Applications:	Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Size:	100 microg
Clone:	2F3
Gene:	3014
Dilutions:	Each lot of this H2A.X antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 microg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer:	Upon receipt, store at 4C.
Concentration:	0.5 mg/ml
Antigen:	Modified peptide
Isotype:	Mouse IgG1, kappa
Clonality:	Monoclonal
Regulatory status:	RUO
Shipping temp:	Ambient RT
Storage temp:	4 C
Alternative names:	H2A.x, H2a/x, Histone 2A, Histone 2A.X