Purified anti-His Tag

Cat no: 652502

Supplier: BioLegend

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Description
Additional Information

This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more). It can also be used for ICFC and IF staining of His-Tagged protein.

Catalogue number:	652502
Hosts:	Mouse
Applications:	Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
Size:	100 microg
Clone:	J099B12
Dilutions:	Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.1-0.2 microg/ml antibody dilution buffer for each mini-gel. Immunofluorescence microscopy, suggested working dilution(s): Use 0.5-1.0 microg/ml antibody dilution buffer. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer:	Upon receipt, store undiluted at 4C.
Concentration:	0.5 mg/ml
Antigen:	KLH-conjugated His-tag peptide
Isotype:	Mouse IgG, kappa
Clonality:	Monoclonal
Regulatory status:	RUO
Shipping temp:	Ambient RT
Storage temp:	4 C
Alternative names:	Histidine Tag