Purified anti-human C3a/C3a(desArg)/C3

C3, complement component 3, is a 195 kD glycoprotein heterodimer that is linked by disulfide bonds. It is the fourth complement component to react in the classical pathway and it is also a reactant in the alternative complement pathway. C3 convertase splits C3 into C3b and C3a. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils have receptors for C3b. C3a is released into the surrounding fluids. It can bind to receptors on basophils and mast cells triggering them to release their vasoactive contents (e.g., histamine). Because of the role of these materials in anaphylaxis, C3a is called an anaphylatoxin. In blood, the C3a fragment is rapidly desarginated by serum carboxypeptidase N enzyme to generate C3a desArg. Both C3a and C3a desArg are not glycosylated.

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SPECIFICATIONS

Catalog Number

518101

Size

50 microg

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum

Clone

K13/16

Antigen

Human C3a

Gene Id

718

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of C3a recombinant protein ranging from 39 to 2500 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

C3a, C3a desarg, C3, complement component 3, complement C3 alpha chain

Regulatory Status

RUO