Purified anti-human IL-12 (p70)

Cat no: 511001

Supplier: BioLegend

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Description
Additional Information

Bioactive IL-12 is a potent regulator of cell-mediated immune responses and key mediator of Th1 cell development. Bioactive IL-12 is secreted by activated B lymphocytes, dendritic cells and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD and 40 kD subunits. The disulfide-linked p40 homodimer can bind to IL-12 receptors and antagonize the activities of bioactive IL-12. The 7B12 antibody reacts with human IL-12 p70 heterodimer, but shows no reactivity with IL-12 p35 or p40 monomer. The 7B12 has high affinity to human IL-12 p70 heterodimer.

Catalogue number:	511001
Reactivities:	Human
Hosts:	Mouse
Size:	50 microg
Clone:	7B12
Gene:	3592,3593
Dilutions:	Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12 p70 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. For use as an ELISPOTcapture antibody, a concentration range of 2-6 microg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer:	The antibody solution should be stored undiluted at 4C.
Concentration:	0.5 mg/ml
Isotype:	Mouse IgG1, kappa
Clonality:	Monoclonal
Regulatory status:	RUO
Shipping temp:	Ambient RT
Storage temp:	4 C
Alternative names:	Interleukin-12 p70, p40 and p35 cystine linked subunits, CLMF, NKSF, TcMF, TSF