Purified anti-human IL-33

IL-33, also known as NFHEV and DVS 27, is a 30 kD proinflammatory protein that belongs to IL-1 superfamily. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1alpha or IL-1beta stimulation. IL-33 interacts with IL1RL1/ST2 receptor and IL-1 receptor accessory protein (IL1RAP) in order to activate intracellular molecules in the NF-kappaB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. Recombinant IL-33 has been shown to enhance Th2-associated immune responses and potently increase mast cell proliferation and cytokine production.

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SPECIFICATIONS

Catalog Number

516301

Size

100 microg

Hosts

Goat

Reactivities

Hum

Clone

Poly5163

Antigen

Human IL-33, amino acids Ser112-Thr270 (Accession# NM_ 033439) was expressed in E. coli.

Isotype

Goat Ig

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 0.25-1 microg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-33 recombinant protein ranging from 1000-15.6 pg/ml are recommended.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C. Do not freeze.

Regulatory Status

RUO