Purified anti-human IL-8

IL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. The E8N1 antibody recognizes the human IL-8 protein and has been shown to be useful for intracellular immunofluorescence flow cytometric analysis and as ELISA detection antibody.

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SPECIFICATIONS

Catalog Number

511402

Size

500 microg

Applications

ELISA

Hosts

Mouse

Reactivities

Hum

Clone

E8N1

Gene Id

3576

Isotype

Mouse IgG1, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1.0 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-8 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

Interleukin-8, NAP-1, MDNCF, NAF, LAI, GCP, CXCL8

Regulatory Status

RUO