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Purified anti-human/mouse/rat PCNA

Purified anti-human/mouse/rat PCNA

Cat no: 307901


Supplier: BioLegend
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The PC10 monoclonal antibody reacts with proliferating cell nuclear antigen also known as PCNA or the DNA polymerase delta auxiliary protein. PCNA is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase delta processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody.
Catalogue number: 307901
Reactivities: Human, Mouse, Rat
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 25 microg
Clone: PC10
Gene: 5111
Dilutions: Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. Please follow theCell Fixation and Permeabilization Protocol Using 70% Ethanol.For immunofluorescent staining, the suggested use of this reagent is ?0.125 microg per million cells in 100 microl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: The antibody solution should be stored undiluted at 4C.
Concentration: 0.5 mg/ml
Antigen: Recombinant rat PCNA
Isotype: Mouse IgG2a, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Proliferating Cell Nuclear Antigen, DNA Polymerase delta Auxiliary Protein
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