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Purified anti-human TNF-alpha

Purified anti-human TNF-alpha

Cat no: 502801


Supplier: BioLegend
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TNF-alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-alpha. Monomeric human TNF-alpha is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-alpha forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-alpha has also been described. TNF-alpha binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The MAb1 antibody can neutralize the bioactivity of natural or recombinant TNF-alpha.
Catalogue number: 502801
Reactivities: Human
Hosts: Mouse
Applications: Western Blot
Size: 50 microg
Clone: MAb1
Gene: 7124
Dilutions: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.25-1 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of TNF-alpha recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: The antibody solution should be stored undiluted at 4C.
Concentration: 0.5 mg/ml
Antigen: E. coli-expressed, recombinant human TNF-alpha
Isotype: Mouse IgG1, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Tumor necrosis factor-alpha, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
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