Purified anti-IkB-alpha

IkappaB-alpha isan inhibitory protein that binds the NFkappaB complex and retains this complex in the cytoplasm where it cannot activate transcription. Upon cellular stimulation, the IkappaB proteins are phosphorylated and degraded via the ubiquitin pathway allowing nuclear translocation of NFkappaB. The NFkappaB complex binds DNA to activate gene expression of a number of a variety of proteins involved in inflammation, cellular proliferation, differentiation, and apoptosis. Poly6249 recognizes the C-terminal region of human, mouse, rat IKB-alpha and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.

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SPECIFICATIONS

Catalog Number

624901

Size

50 microl

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Clone

Poly6249

Antigen

Peptide mapping to the carboxy terminus of human IkappaB-alpha

Gene Id

4792

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

4 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C. DO NOT FREEZE.

Alternative Names

Nuclear factor of kappa light chain enhancer in B cells inhibitor alpha, IKBA

Regulatory Status

RUO