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Purified anti-LAT

Purified anti-LAT

Cat no: 623901

Supplier: BioLegend
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LAT (linker for activation of T-cells) is a 36 kD phospho-tyrosine adaptor protein containing SH2 domains and a CVRC motif. Two LAT isoforms have been reported with approximate molecular weights of 36 kD and 38 kD. LAT is localized to lipid rafts, and is also a potential type III transmembrane protein, thought to be expressed in the juxtanuclear intracellular compartment. LAT links TCR engagement to Ras-MAPK activation, Ca2+ flux, IL-2 expression and is required for T-cell development. LAT is phosphorylated when activated by TCR cross-linking, where it recruits signaling molecules. LAT activation can be prevented by polyunsaturated fatty acids. LAT is modified by ZAP-70 and Syk phosphorylation and can also be palmitoylated. LAT has been reported to be associated with Grb2, Gads, Grap, PLC-gamma1, p85 subunit PI3K, ZAP-70, Syk, Itk, Vav, SLP-76, and cbl. The LAT1111 monoclonal antibody recognizes the cytoplasmic domain of human LAT and has been shown to be useful for Western blotting and immunoprecipitation. The LAT1111 antibody does not cross-react with mouse LAT.
Catalogue number: 623901
Hosts: Mouse
Applications: Immunoprecipitation, Western Blot
Size: 25 microg
Clone: LAT1111
Gene: 27040
Dilutions: Each lot of this LAT antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 microg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store at 4C.
Concentration: 0.5 mg/ml
Antigen: Partial recombinant protein (cytoplasmic domain of human LAT)
Isotype: Mouse IgG1
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Linker for activation of T-cells, 36 kD phospho-tyrosine adaptor protein
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