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Purified anti-mouse/human IL-5

Purified anti-mouse/human IL-5

Cat no: 504302


Supplier: BioLegend
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IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK5 antibody can neutralize the bioactivity of natural or recombinant IL-5.
Catalogue number: 504302
Reactivities: Human, Mouse
Hosts: Rat
Applications: Immunohistochemistry, Western Blot
Size: 500 microg
Clone: TRFK5
Gene: 16191,3567
Dilutions: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-5 recombinant protein ranging from 250 to 1 pg/ml are recommended for each ELISA plate when paired with TRFK4 (Cat. # 504402). To obtain a linear standard curve, serial dilutions of human IL-5 recombinant protein ranging from 250 to 1 pg/ml are recommended for each ELISA plate when paired with JES1-5A10 (Cat. # 501002). It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: The antibody solution should be stored undiluted at 4C.
Concentration: 0.5 mg/ml
Antigen: Partially-purified T cell clone supernatant
Isotype: Rat IgG1, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Interleukin-5, EDF, Eo-CSF, BCGF-2, BCDF-m, IgA-EF, TRF-1
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