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Purified anti-mouse IL-10
BioLegend

Supplier:

BioLegend

Cat no: 505001

Purified anti-mouse IL-10

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. IL-10 inhibits IFN-gamma, TNF-beta, and IL-2 production by Th1 clones; inhibits macrophage-mediated IL-1, IL-6, and TNF-alpha synthesis; suppresses the delayed type hypersensitivity response; stimulates Th2 cell response (which results in elevated antibody production); and promotes mast cell proliferation in combination with IL-4.

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SPECIFICATIONS

Catalog Number

505001

Size

50 microg

Applications

IHC

Hosts

Rat

Reactivities

Mouse

Clone

JES5-16E3

Antigen

E. coli-expressed, recombinant mouse IL-10

Gene Id

16153

Isotype

Rat IgG2b, kappa

Clonality

Monoclonal

Dilutions

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.1-1.0 microg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. For ELISA capture, Carbonate buffer, pH 9.5 is highly recommended for coating buffer.

Storage Temp

4 C

Concentration

0.5 mg/ml

Shipping Temp

Ambient RT

Storage Buffer

The antibody solution should be stored undiluted at 4C.

Alternative Names

Interleukin-10, CSIF, B-TCGF, TGIF

Regulatory Status

RUO

SUPPLIER INFO

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

More info

Applications

FC, ICC

Hosts

Mouse

Reactivities

Hum

More info

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