Purified anti-NEK9

NEK9 is a 107 kD member of the serine/threonine-protein kinase family, NIMA subfamily containing a coiled-coil domain, and RCC1 repeats. This cytoplasmic protein forms dimers and is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation, Nek9 is activated during mitosis by intramolecular autophosphorylation at Thr210. Nek9 has been shown to interact with Ran GTPase (greater affinity for Ran-GDP over Ran-GTP), NEK6, NEK7, BICD2, histone H3, myelin basic protein, and beta-casein. The Poly6241 antibody recognizes human NEK9 and has been shown to be useful for Western blotting, immunohistochemistry, and immunoprecipitation.

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SPECIFICATIONS

Catalog Number

624102

Size

200 microl

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Clone

Poly6241

Antigen

Partial recombinant protein, responding to the middle region of NEK-9

Gene Id

91754

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store frozen at -20 C.

Alternative Names

Serine/Threonine-protein kinase Nek9, NimA-related protein kinase 9

Regulatory Status

RUO