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Purified anti-Neurofilament heavy protein (NF-H)

Purified anti-Neurofilament heavy protein (NF-H)

Cat no: 626402

Supplier: BioLegend
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NF-H is an abundant, stable cytoplasmic protein located in neuronal cells in large axons frequently used as a cell type marker. The NF-H protein shares a high degree of structural and sequence homology with the NF-L and NF-M subunits, especially in the coiled-coil core domain. NF-M and NF-H form flexible extensions linking the neurofilament proteins to each other and other cytoplasmic proteins. Deletions and insertions in the NF-H protein have been reported in amyotrophic lateral sclerosis. The NF-01 monoclonal antibody reacts with a conserved phosphorylated epitope of NF-H present in all species and has been reported to be useful for Western blotting, immunohistochemistry using formalin-fixed, paraffin-embedded tissues and immunofluorescence staining.
Catalogue number: 626402
Hosts: Mouse
Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 100 microg
Clone: NF-01
Gene: 380684
Dilutions: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 10 microg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store undiluted at 4C.
Concentration: 0.5 mg/ml
Antigen: Pellet of pig brain cold stable proteins after microtubule depolymerization
Isotype: Mouse IgG1
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Neurofilament protein, heavy polypeptide; neurofilament heavy polypeptide
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