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Purified anti-Nucleolin-Phospho (Thr76/Thr84)

Purified anti-Nucleolin-Phospho (Thr76/Thr84)

Cat no: 609403

Supplier: BioLegend
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Nucleolin is a ubiquitously expressed 100 kD nuclear and nucleolar protein that contains RNA recognition motifs. Nucleoloin is the major nucleolar protein of eukaryotic cells in growth phase associated with intranucleolar chromatin and pre-ribosomal particles. This protein induces chromatin de-condensation by binding to histone H1 and plays a role in pre-rRNA transcription and ribosome assembly. Nucleolin exhibits self-cleaving, DNA helicase, RNA helicase and DNA-dependent ATPase activities; activity can be upregulated in response to ERK signaling, c-Myc expression, and genotoxic stress (UV radiation). This protein is modified by phosphorylation (Thr76/Thr84). This protein interacts with phosphatase 1delta, hepatitis C virus NS5B, part of B-cell specific transcription factor complex with LR1, and DNA Topoisomerase I. The 10C7 monoclonal antibody recognizes human phosphorylated nucleolin (Thr76/Thr84) and has been shown to be useful for Western blotting.
Catalogue number: 609403
Reactivities: Human
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 25 microg
Clone: 10C7
Gene: 4691
Dilutions: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 microl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use 10 microg/ml of antibody for staining. Antigen retrieval using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: Upon receipt, store at 4C. Do not freeze.
Concentration: 0.5 mg/ml
Antigen: Modified peptide
Isotype: Mouse IgM, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Nucleolin protein, C23
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