Purified anti-PERK Phospho (Ser713)

PERK (protein kinase-like endoplasmic reticulum kinase) is a type I membrane protein located in the endoplasmic reticulum (ER). ER stress increases the activity of PERK, which then phosphorylates eIF2alpha to promote reduced translation, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. PERK-deficient mice have defects in pancreatic beta cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress. PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain. Phosphorylation of PERK at Ser713 can be used as a marker for its activation status. The predicted phosphorylation site and surrounding residues of Ser713 are: HIEIIAPS*PQRSRSF.

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SPECIFICATIONS

Catalog Number

649401

Size

100 microl

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Clone

Poly6494

Antigen

Modified synthetic peptide

Gene Id

9451

Isotype

Rabbit Polyclonal

Clonality

Polyclonal

Dilutions

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 20 microl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Storage Temp

-20 C

Concentration

Shipping Temp

Ambient RT

Storage Buffer

Upon receipt, store at -20C.

Alternative Names

eukaryotic translation initiation factor 2 alpha kinase 3; PRKR-like endoplasmic reticulum kinase; heme-regulated EIF2-alpha kinase

Regulatory Status

RUO