Purified anti-Phosphotyrosine

Cat no: 309301

Supplier: BioLegend

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Description
Additional Information

Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.

Catalogue number:	309301
Hosts:	Mouse
Applications:	Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Size:	25 microg
Clone:	PY20
Gene:	5800
Dilutions:	Western blotting, suggested working dilution(s): Use1-2 microg per ml antibody dilution buffer per mini-gel. Do not use dilution or blocking buffers containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer:	The antibody solution should be stored at -20C.
Concentration:	0.5 mg/ml
Antigen:	KLH-conjugated phosphotyrosine
Isotype:	Mouse IgG2b, kappa
Clonality:	Monoclonal
Regulatory status:	RUO
Shipping temp:	Ambient RT
Storage temp:	-20 C